Pdf engineered resistance to nosema bombycis by in vitro. In this study, a singlechain variable fragment scfv. Early and simultaneous detection of nosema bombycis microsporidia. Infection and development of nosema bombycis microsporida. The microsporidian nosema bombycis is an obligate intracellular pathogen of the silkworm bombyx mori, causing the epidemic disease. Article information, pdf download for laser tweezers raman. Easy labeling of proliferative phase and sporogonic phase of microsporidia nosema bombycis in host cells plos one, dec 2019 jie chen, wei guo, xiaoqun dang, yukang huang, fangyan liu, xianzhi meng, yaoyao an, mengxian long, jialing bao, zeyang zhou, et al. Pdf influence of temperature and relative humidity in infection of. Identification of a nosema bombycis microsporidia spore wall. Nosematidae, nucleopolyhedrovirus baculoviridae, and densovirus parvoviridae by multiplex realtime polymerase chain reaction in bombyx mori lepidoptera. Microsporidia are a group of obligate intracellular parasites with widespread global distribution. Pdf the influence of temperature and relative humidity in infection and crossinfection of nosema bombycis and n. Identification of a nosema bombycis microsporidia spore wall protein corresponding to spore phagocytosis volume 8 issue 9 shunfeng cai.
Nosema bombycis microsporidia suppresses apoptosis in bmn. Pdf a monoclonal antibody to nosema bombycis and its use. Neither fragment hybridized with genomic dna from four other microsporidian isolates tested. Quantitative proteomic analysis of germination of nosema. Pdf a specific molecular label for identifying mature.
A hybridoma cell line secreting monoclonal antibodies mab to spore surface antigens of nosema bombycis was prepared by fusion of myeloma cells with. Nosema bombycis, a microsporidium, is a pathogen of pebrine disease of silkworms, and its genomic dna sequences had been determined. A specific molecular label for identifying mature nosema. Nosema bombycis is a destructive, obligate intracellular parasite of the bombyx mori. The genomic sequences for both silkworm bombyx mori and microspo ridia nosema. Engineered resistance to nosema bombycis by in vitro expression of. A monoclonal antibody to nosema bombycis and its use for identification of microsporidian spores. Laser tweezers raman spectroscopy ltrs to detect effects of.
The microsporidium nosema bombycis nb causes pebrine, a fatal disease in. Spores of nosema bombycis derived from diseased insects were highly purified by urografin density gradient centrifugation. Microsporidia can survive outside the host for several. Easy labeling of proliferative phase and sporogonic phase. Early and simultaneous detection of nosema bombycis.
Their host range in invertebrates and vertebrates includes insects, fishes and humans cali and takvorian, 1999, didier et al. Pdf the influence of temperature and relative humidity in infection and cross infection of nosema bombycis and n. Two dna fragments which hybridize specifically with dna of nosema bombycis and nosema costelytrae, respectively, were obtained from genomic dna of each microsporidian species and sequenced. Nosema bombycis, a parasite of the silkworm bombyx mori, was the first microsporidian species identified and causes pebrine disease. Nosema bombycis, the first reported microsporidium, is a type of obligate intracellular parasite that infects silkworms and can be induced to germinate in alkaline solution in vitro. A monoclonal antibody to nosema bombycis and its use for.
Pdf pebrine is a deadly disease of mulberry silkworm, bombyx mori l. Nose ma bombycis, the microsporidia first described in the literature, is a silkworm parasite that causes severe eco nomic losses in the sericulture industry. Nosema bombycis spore preparation and infection of cells. Morphology and transcriptome analysis of nosema bombycis. Bombycidae volume 149 issue 2 shan wu, yongqiang he, xingmeng lu, xiaofeng zhang, jiangbing shuai, huizhen yu, chenchen jin, hongfei mo.
358 988 186 1622 982 1001 1057 927 2 670 544 1268 615 1493 276 1507 486 1511 1071 226 545 853 231 1101 1041 265 454 1398 1570 1472 132 774 1524 1067 57 574 1323 1133 1014 1066 1145 309